A Bacterial Enzymatic Method for Determining Tyrosine in Cheese
نویسندگان
چکیده
منابع مشابه
Kinetic enzymatic method for determining serum creatinine.
Creatinine amidohydrolase is used to measure serum creatinine in a totally enzymatic procedure. Creatine, produced by hydrolysis, is acted upon by creatine kinase, and then by pyruvate kinase and lactate dehydrogenase, to result in a change in absorbance at 340 nm. The amount of creatinine present is related to the rate of change in A340 and is determined from a standard curve. Absorbance and c...
متن کاملEvaluation of an enzymatic method for determining creatinine in plasma.
An enzymatic kit method for the determination of plasma creatinine was optimised for use with a centrifugal analyser and its performance characteristics and practicability compared with an end point and a kinetic Jaffé-based method. The enzymatic method exhibited several advantages over Jaffé-based methods--namely, smaller sample size, rapid sample throughput (200 per hour), and improved specif...
متن کاملNew enzymatic method with tryptophanase for determining potassium in serum.
We established a simple and rapid enzymatic method for measuring potassium ion in serum by using tryptophanase (EC 4.1.99.1) purified from Escherichia coli K12 strain (E. coli K12 IFO 3301). The presence of pyridoxal 5-phosphate promotes this enzymatic reaction, and potassium and (or) ammonium ions further accelerate it, with ammonium and potassium ions providing equivalent acceleration. We eli...
متن کاملManual fluorometric paper disc method for determining tyrosine in blood.
A spectrophotofluorometric method is described for the determination of tyrosine in blood dried on filter paper discs. It has been found that by immersing the paper disc containing the dried blood in aqueoustrichioracetic acid, the blood proteins remain bound to the paper and the amino acid is eluted into the aqueousphase.This method of assay is based on the principle that tyrosine reacts with ...
متن کاملEMDee: an enzymatic method for determining enantiomeric excess.
The techniques of combinatorial chemistry have recently been applied to the discovery of new asymmetric catalysts for a variety of organic transformations.1 These techniques are useful for increasing the efficiency by which chiral ligands for metal catalysts are discovered since the design of these ligands is largely an empirical process, and there are currently no simple rules for reliably pro...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1955
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(55)95062-1